Research Publications

ZITTE, Leelee Famii

  1. Author:  KONYA, Roseline Sonayee, ZITTE, Leelee Famii and CHUKWU, Kenneth Obinna

Title:  IN VITRO EVALUATION OF THE EFFECT OF Euphorbia kamerunica LATEX ON THE BLOOD OF ALBINO RAT (Rattus norvegicus) - Animal Research International (2013) 10(3): 1747 – 1751

Link: http://search.proquest.com/openview/3991fceb63e2b72dab5963155a3894c1/1?pq-origsite=gscholar

Abstract: The effect of Euphorbia kamerunica latex on the blood of albino rat (Rattus novergicus) was studied. Two different samples were prepared, one with 10% E. kamerunica latex in blood of albino rat and the other, whole blood. Blood from each of the two samples was analyzed haematologically for the packed cell volume (PCV), haemoglobin (Hb), red blood cell (RBC), white blood cell (WBC) and neutrophils. The results of the study showed that there was a drop in all the blood parameters in the blood sample with the latex as compared with the whole blood. The PCV dropped from 17% to 9%, (47.06% reduction), Hb reduced from 5.1g/dl to 3.2g/dl (37.25% reduction), RBC had a three log reduction from 3.7x1012 to 2.7x109 (99.88% reduction), WBC fell from 4.2x109 to 3.2x107 (99.24 % reduction) while neutrophils dropped from 20% to 15% (25.00% reduction) in the blood with latex. The proximate analysis of the latex revealed that it contains 62.13 ± 0.48 % lipid, 0.16 ± 0.01 % carbohydrate, 3.69 ± 0.05 % protein and 3.86 ± 0.24 % ash. The result of this in vitro study demonstrated that Euphorbia kamerunica has some toxic effects on the blood of Rattus novergicus.

 

Keywords: Euphorbia kamerunica, Latex, Rattus norvegicus, Packed cell volume, Haemoglobin, Red blood cell, White blood cell, Neutrophils

 

  1. Author:  Michael N. WOGU, Florence O. NDUKA and MacDonald D. WOGU

Title: Prevalence of Malaria Parasite Infection among Pregnant Women Attending Antenatal Clinics in Port Harcourt, Rivers State, Nigeria - International Journal of TROPICAL DISEASE & Health 3(2): 126-132, 2013

Link: http://imsear.li.mahidol.ac.th/handle/123456789/153218

Abstract: Aims: To evaluate the prevalence of malaria parasites among some pregnant women attending antenatal clinics in Rivers State, Nigeria. Study Design: Cross-sectional study. Place and Duration of Study: Five healthcare centres in Rivers State, Nigeria, between April and September 2011. Methodology: Peripheral blood samples were collected using venous procedure and the presence of malaria parasites was observed microscopically on thick and thin blood smears prepared from each sample. Personal data were collected through questionnaires and the general results gotten during this study were analyzed statistically using two-way Analysis of Variance (ANOVA). Results: The only species of malaria parasite identified in this study was Plasmodium falciparum. A total of 104 (26%) pregnant women were infected with P. falciparum in this study. Pregnant women in their first, second and third trimesters had prevalence rates of 27%, 27.3% and 21.8% respectively (P<0.05). Prevalence rates for primigraviidae, secundigraviidae and multiparous women were 26.1%, 31.5% and 20% respectively (P<0.05). prevalence rates of 26.9%, 27.8% and 21.5% were observed in pregnant women between the ages of 11-20, 21-30 and 31-40 respectively (P<0.05). Conclusion: Malaria still needs to be given more attention due to its negative impact on pregnant women and their unborn children.

 

Keywords: Prevalence; malaria; pregnancy; women; Plasmodium; gestation period; parity; age.

 

  1. Author:  Michael N. Wogu, Florence O. Nduka and MacDonald D. Wogu

 

Title: Effectiveness and Compliance of Long Lasting Insecticide Nets (LLINs) on Malaria Parasitemia among Pregnant Women attending Antenatal Clinics in Port Harcourt, Rivers State - British Journal of Medicine and Medical Research, ISSN: 2231-0614,Vol.: 3, Issue.: 4 (October-December)

Link: http://sciencedomain.org/abstract/1184

Abstract: Aim: To determine the effectiveness and compliance of Long Lasting Insecticide Nets (LLINs) on malaria parasitaemia among pregnant women attending antenatal clinics.

Study Design: Cross sectional study.

Methodology: Thick and thin blood films were made and stained using parasitological standard procedures to identify malaria parasites. Questionnaires were distributed to collect personal data of the pregnant women examined. Data gotten was analyzed with Chi-square test of significance.

Results: A total of 400 pregnant women were examined, 317(79%) used LLINs with 29(9.1%) positive for malaria parasites while 83(21%) did not use LLINs with 75(90.4%) positive for malaria parasites (P<0.05). Pregnant women in their first, second and third trimesters that used LLINs had prevalence rates of 5.3%, 12.2% and 11.5% respectively while those that did not use LLINs had prevalence rates of 89.1%, 94.1% and 90.9% respectively (P<0.05). Primigraviidae, secundigraviidae and multiparous women who used LLINs had prevalence rates of 10.3%, 8.7% and 5.9% while those who did not use LLINs had prevalence rates of 95.8%, 89.5% and 75% respectively (P<0.05).

Conclusion: The usage of LLINs in reducing malaria parasitaemia among pregnant women was statistically significant (P<0.05) irrespective of parity and gestation period.

Keywords: Prevalence; malaria; pregnancy; women; trimester; parity; LLINs.

 

  1. Author:  S. O. Nzeako, F. O. Nduka, M. N. Wogu and J. I. Monye

Title: Isolation of Parasites from Black Tiger Prawn; Peneaus monodon from Isaka River, Okirika Local Government Area, Rivers State, Nigeria - Annual Research & Review in Biology 8(1): 1-11, 2015, Article no.ARRB.19302

Link: https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwiX2O-9yuTNAhVpIMAKHa5cBmEQFggeMAA&url=http%3A%2F%2Fsciencedomain.org%2Fdownload%2FMTA1NzdAQHBm&usg=AFQjCNFMXVTjdMmh3avc17UQkrRn-2O8GQ

Abstract: Aims: To determine the parasitic fauna, site-specificity of the parasites in the host and the parasite load in relation to length, weight and sex of P. mondon. Study Design: The study is a survey using prawns from Isaka River as a case study.

Methodology: Cast and spread technique was adopted in the study. Fishing-net was used to collect samples from the River. The stratified random sampling method was used in selecting the 103 prawns examined in the study. The hemolymph of the prawn was extracted using a 2 ml syringe at the site of collection to maintain the integrity of the specimen and preserved in EDTA bottle. Samples were then preserved in an ice chest and transported to the laboratory for morphometric and growth parameters evaluation. Exo-secretions, gills, appendages and gastrointestinal contents of the prawns were evaluated using standard parasitological techniques. Identification of parasites was achieved using the compound light microscope and standard guides. Physiochemical parameters of the River were determined using standard techniques and equipment. Data was analyzed with Measures of Central tendencies and Analysis of Variance.

Results: The data revealed an overall prevalence of 97.09% in the study. However, females harbored (58%) more parasites than males (42%). Parasites belonging to four phyla; Nematoda 111(17.03%), Platyhelminthes 32(4.08%), Arthropoda 73(10.63%) and Protozoa 469(68.27%) were recovered from the study. Phylum Protozoa had the highest abundance of 475(68.27%) and had the highest parasite diversity of up to eight species. Faunal specificity revealed a total of 742 parasites comprising of (11.05%), Trichuris spp. (4.16%), Ascaris spp. (2.15%), Spirocamallanus spp. (2.82%), Hysterothylacium spp. (1.62%), Capillaria spp. (0.80%), Enterobius spp. (4.17%), Lernaea (3.23%), Trematode (5.64%), Nematopsis spp. (9.7%), Porospora spp. (11.96%), Haplosporidium spp. (11.29%), Blastodinium spp. (11.02), Vahlkampfia spp. (3.36%), Colacium

spp. (5.64%), Paramoeba spp. (6.04%), Pekinsus spp. (3.62%), Tapeworm (1.21%), Diatoms (4.56%) and Myzomolgus spp. (6.61%) were recovered. There were significant differences (P>0.05) in parasite loads in the examined animals in relation to the sex, body length, and weight. Site specific parasitism showed that the hemolymph had the highest parasite load of (80.58%) while the gastrointestinal tract had the highest diversity of parasites in the study. The physiochemical parameters of the water body varied from standards permissible in brackish water ecosystem.

Conclusion: The study revealed that P. monodon was highly susceptible to a wide range of parasites, attributable to the compromised ambient environmental status of the habitat and trophic affiliation of the prawns. The study states that the lipoproteins enriched hemolymph of P. monodon may have predisposed it to heavy parasitism.

 

Keywords: Exo-secretions; P. monodon; site specific parasitism; diversity of parasites; environmental health status.

 

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