Research Publications

A E Arinze

Storage rot of sweet potato (Ipomoea batatas) and the effect of fungicides on extracellular cellulolytic and pectolytic enzymes of the causal organism.  [1975]

Arinze A.E. Naqvi S.H.Z. Ekundayo J.A.

No abstract

http://agris.fao.org/agris-search/search.do?recordID=CZ19760051327

 

 

Changes in phenol oxidase and peroxidase levels in cocoyam tubers of different postharvest ages infected by Sclerotium rolfsii sacc

 

    Dr. N. C. Ohazurike* andA. E. Arinze

 

Version of Record online: 19 OCT 2006

 

DOI: 10.1002/food.19960400107

Abstract

 

Crude aqueous extracts from the peripheral rot zone of cocoyam tubers infected by Sclerotium rolfsii sacc were shown to be inhibitory to dialysed in vivo polygalacturonase (PG) of the pathogen. The PG inhibitory action, phenol oxidase and peroxidase activities were higher in cocoyam tubers of the Xanthosoma sagittifolium varieties than in those of the Coolocasia esculenta varieties. The levels of phenol oxidase, peroxidase and PG inhibitory activities also decreased as the postharvest age of the tubers increased.

http://onlinelibrary.wiley.com/doi/10.1002/food.19960400107/abstract

 

 

EVALUATION OF GROWTH OF SOME FUNGI IN CRUDE OIL POLLUTED ENVIRONMENT

CI Onuoha, AE Arinze, AE Ataga

 

Abstract

 

The vegetative growth response of some fungi to different concentrations of crude oil was investigated. The result showed a variable degree of sensitivity to crude oil. The radial growth of Rhizopus sp. was not affected by the oil irrespective of the concentration. The two species of Aspergillus A. niger and flavus used in the study appreciably tolerated the oil though the growth of A. flavus was retarded as the concentration of oil increased. Botrytis cinerea, Curvularia lunata and Colletotrichum sp. could not grow beyond the concentration of 1ml/10mls of oil Potato Dextrose Dextrose Agar (PDA). The three fungi could serve as sensitivity index for monitoring oil pollution in an environment.

KEY WORDS: Crude oil, pollution, fungal growth.

Global Journal of Agricultural Sciences Vol.2(2) 2003: 80-81

http://www.ajol.info/index.php/gjass/article/view/2210

 

 

Distribution of polygalacturonase, total phenolic substances, polyphenol oxidase and peroxidase in rot zones in sweet potato

  1. A. E. ARINZE* and
  2. I. M. SMITH

Version of Record online: 5 APR 2007

DOI: 10.1111/j.1365-3059.1982.tb02819.x

Botryodiplodia theobromae causes a spreading rot of sweet potato, while Botrytis cinerea, Phoma exigua var. foveata and Fusarium solani var. coeruleum cause rots of limited extent. Cladosporium cucumerinum does not rot sweet potato tuber tissue. Whichever of these fungi is inoculated into tubers, a zone at the margin of the lesion can be clearly distinguished, from slightly affected but uninfected tissue on one side and infected rotted tissue on the other, by its high content of phenolic substances and polyphenol oxidase and peroxidase activities. Polygalacturonase is present in this zone for spreading B. theobromae lesions but not for the limited lesions caused by the other fungi. Total content of phenolic substances is generally greater in and around limited lesions.

http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3059.1982.tb02819.x/abstract

 

 

Production of extracellular cellulolytic and pectic enzymes by Lasiodiplodia theobromae on sweet potato (Ipomoea batatus) tubers  [1976]

Arinze, A.E. Naqvi, S.H.Z. Ekundayo, J.A.

No abstract

http://agris.fao.org/agris-search/search.do?recordID=US201302993742

 

 

In vitro Seed-dressing Technique for the Control of Seed-borne Fungi of Rice variety Faro -29

OFA Ibiam, CI Umechuruba, AE Arinze

2008

Abstract

 

Use of seed-dressing fungicides (Bavistin, Benlate, Fernasan-D, Apron Plus 50 DS and Dithane–M45), and soaking and slurry methods at various concentrations, for the control of seed-borne fungi of rice variety
Faro 29 in vitro, was investigated. The results obtained showed that all the fungicides significantly inhibited the seed-borne fungi associated with the seeds of the variety at concentrations of 40g/ml, and 50mg/ml (P<0.05), in the soaking method, and at all the concentrations in the slurry method (P<0.05).The mean percentage seed germination , was not below 71%,at all the concentrations in the two methods used . The relevance of this work to the production of rice and food security in Nigeria is discussed.

http://www.ajol.info/index.php/jasem/article/view/55490

 

 

Effects of amendments and bioaugumentation of soil polluted with crude oil, automotive gasoline oil, and spent engine oil on the growth of cowpea (Vigna ungiculata L. Walp)

Adedokun, Olutayo M. and Ataga, A. E.

Accepted 26 April, 2007

The effects of sawdust and waste cotton as soil amendment and bioaugumentation with Pleurotus pulmonarius (pp) on soil polluted with crude oil (COIL), automotive gasoline oil (AGO), and spent engine oil (SEO) on the growth of cowpea (Vigna ungiculata L. Walp) was investigated. There was a significant improvement (P = 0.05) on the growth of cowpea when polluted soil was amended and bioaugmented with P. pulmonarius (pp) after one month of incubation when compared with the result of planting on polluted soil with no amendments and bioaugumentation. Addition of waste cotton as an amendment and P. pulmonarius as bioaugumentation agents to crude oil polluted soil significantly reduced time of seed germination from 8 to 3 days, increased seed germination from 60 to 96%, plant height from 10.3 to 22 cm, leaf number from 3 to 5 and biomass from 0.5 to1.5 g dry wt. Similar reductions in time of germination, increases in percentage germination, plant height, leaf number and total biomass in cowpea plants grown in automotive gasoline oil and spent engine oil polluted soils, amended with waste cotton or saw dust and bioaugumented with P. pulmonarius were observed in this study.

 

Key words: Amendments, bioaugumentation, Pleurotus pulmonarius, Vigna and growth.

http://www.academicjournals.org/article/article1380188654_Adedokun%20and%20Ataga.pdf

 

 

Determination of in vitro and in vivo production of polygalacturonase (pg) by storage mold aspergillus niger v. Tieghem, during storage of rice (oryzae sativa l) seeds

OFA Ibiam, AE Arinze

2007

Abstract

 

The in vitro and in vivo production of pectic enzyme polygalacturonase (PG) by storage mold of rice Aspergillus niger and the effect of temperature and pH on the activity of the mold were investigated. The
result of the assay for the production of polygalacturonase (PG) by the mold in vitro and in vivo showed that the activity of the enzyme when tested viscometrically, using 10% pectin was 142.9 R.V.U (Relative viscometric units) at 25 oC. The optimum activity of the enzyme secreted in vitro by Aspergillus niger was obtained at a temperature of 25 oC, and at temperature of 25 oC and 30 oC for that secreted in vivo., with a value of 142.9 R. V.U. The least activity of the enzyme was obtained at 20 oC, 40 oC, 45 oC and 50 oC in vitro, and at 20 oC ,45 oC and 50 oC with a value of 111.1 R.V.U each. The optimum activity of the enzyme produced in vitro by the fungus obtained at pH 5 and 6 with a value of 125R.V.U, and at pH 5 with a value of 142.9 R .V. U. in vivo. The activity of the enzyme was least at pH 2, 3, 4, 7,8, 9 and 10 in vitro, and at pH 2,3,8,9 and 10. in vivo with a value of 111.1 R. V. U.

http://www.ajol.info/index.php/jasem/article/view/55000

 

 

Title:

Studies on the storage rot of sweet potato (Ipomoea batatas L. & Lam.) by Botryodiplodia theobromae Pat. and other fungi

 

Author:

Arinze, Anthony Elue

1978

 

No abstract

http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.448071

 

 

The Adsorption of a Polygalacturonase Isoenzyme of Botryodiplodia theobromae on Plant Tissues and the Implication on the Pathogenicity of the Fungus

  1. A. E. Arinze

Version of Record online: 1 MAY 2008

DOI: 10.1111/j.1439-0434.1985.tb04333.x

Abstract

The polygalacturonase isoeazyme (PG 3) of Botryodiplodia theobromae extracted from rotted sweet potato was adsorbed by sweet potato, potato, carrot, bean stem and tomato fruit to various degrees. Adsorption was greater with sweet potato and tomato fruit tissues. Carrots, bean stem and potato absorbed the enzyme to more or less the same degree. The enzyme was not adsorbed on tomato stem. A spore/mycelial suspension of B. theobromae infected the test tissues to various degrees. The enzyme completely macerated sweet potato roots, potato tubers and tomato fruits within 5 h while the bean stem and onion tissues were little affected by the enzyme. The tomato stem was neither infected by the fungus nor macerated by the enzyme.

http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.1985.tb04333.x/abstract

 

 

In vitro and in vivo production of pectic enzymes, polygalacturonase, by seed-borne pathogen, fusarium moniliforme sheldon from seeds of rice (Oryzae sativa L) and its role in the disease of rice

OFA Ibiam, AE Arinze

 

Abstract

 

Possible in vitro and in vivo production of pectic enzyme polygalacturonase and pH on the activity of the enzyme were investigated. The results of the assay for the production of polygalacturonase (PG) by the pathogen showed that the activity was 142.9 RVU in vitro and 166.7 RVU, in vivo at temperature 25°C. The optimum activity of the enzyme in vitro was obtained at 25° C and 30°C, with the value of 142.9 RVU, and the least was at 20°C, 45°C and 50°C, with a value of 111.1 RVU. In vivo, the optimum activity was at 20oC, with a value of 166.7 RVU, while the least activity was 111.1 RVU at 50°C. The activity of PG in vitro was optimum at pH 5 and 6, with a value of 142.9 RVU each, while the least activity was obtained at pH 2, 9 and 10, with a value of 111.1 RVU. During the in vivo studies, the activity of PG was optimum at pH 6 with a value of 166.7 RVU and least at pH 2, 9 and 10, with the value of 111.1 RVU.

Bio-Research Vol. 3(1) 2005: 9-13

http://www.ajol.info/index.php/br/article/view/28563

 

 

Effects of the amendment of crude – oil polluted soil on fungal population

C. I. Onuoha, A. E. Arinze, E. A Ataga

 

Abstract

 

The population of fungal propagules in crude oil polluted and amended soil under simulated condition was investigated. The result showed an initial decrease in the population of fungal propagules, then a rapid increase and finally, a gradual reduction in the number of propagules. An exception to this was in sawdust-amended soil that recorded a rapid increase in fungal population from the beginning up to the 4th week. The polluted and unremediated soil showed a comparatively low fungal population up to the 3rd week before a gradual increase. The soil amended with wood ash showed a comparatively low population of fungal propagules throughout the study.



Keywords: Crude oil, pollution, soil amendment, fungal population

[Global Jnl Environ Sci Vol.2(2) 2003: 96-97]

http://www.ajol.info/index.php/gjes/article/view/2413

 

 

The Action of Polygalacturonase and Cellulase Enzymes of Botryodiplodia theobromae Pat. on Yam (Dioscorea spp.) and Sweet Potato (Ipomoea batatas) Tissues

  1. A. E. Arinse

Version of Record online: 1 MAY 2008

DOI: 10.1111/j.1439-0434.1985.tb00847.x

Abstract

The action of an endo-polygalacturonase PG3 and cellulase of Botryodiplodia theobromae, in the crude and pure forms, on sweet potato and yam slices was investigated. The purified isoenzyme PG3 and the crude pectic enzyme preparations macerated and killed the cells of both tissues although the former did so at a faster rate. Purified cellulase neither macerated nor killed cells from either plant. Although the action was delayed, the crude preparation of cellulase slightly macerated and killed the cells of sweet potato. Yam tissue was, however, not macerated by crude cellulase.

Plasmolysis of the cells of both tissues delayed the toxicity and macerating action of the pure PG3 on the tissues. This delay in cell death was, however, not observed when the plasmolysed cells were treated with the crude cellulase preparation.

http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0434.1985.tb00847.x/abstract

You are here: Home Publications publication-col1 Research Publications A E Arinze